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Recombinant Monkeypox Virus A35R Protein, C-Tana tohu

Whakaahuatanga Poto:


Taipitopito Hua

Tohu Hua

Nga korero whanui

Puna

Virus Monkeypox(te riu Zaire-96-I-16)

Kaihautu Whakaaturanga

HEK 293 Pūtau

Tūtohu

C-Ko tana tohu

Taupānga

He pai mo te whakamahi i nga immunoassays.

Me whakatau e ia taiwhanga he tohu mahi tino pai mo te whakamahi i roto i ana mahi.

Nga korero whanui

Ko te pūmua A35R huaketo monkeypox recombinant ka hangaia e te punaha korero Mammalian me

ka whakaatuhia te ira ira e whakawaehere ana i a Arg58-Thr181 me tana tohu-tohu i te C-terminus.

Āhuatanga

Maamaa

>95% i whakatauhia e SDS-PAGE.

Āhuatanga

Papatipu ngota

Ko te pūmua A35R huaketo monkeypox recombinant kei roto i te 139 waikawa amino me te papatipu ngota o te 15.3 kDa.Ka heke te pūmua hei 15-26 kDa i raro i te whakaiti i te SDS-PAGE na te glycosylation.

Kaipupuri Hua

20 mM Tris, 10 mM NaCl, pH 8.0.

Rokiroki

Whakaorangia i raro i nga tikanga parakore i te -20 ℃ ki te -80 ℃ ina whiwhi.

Ka tūtohu kia tohatohahia te pūmua ki nga rahinga iti mo te rokiroki pai.

Nga korero ota

Ingoa Hua ngeru.Kao Te nui

Recombinant Monkeypox Virus A35R Protein, C-Tana tohu

AG0090

Whakaritea


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