Recombinant Tangata CEACAM5 Pūmua, C-Tana tohu

Taipitopito Hua

Puna	Te pūtau whatukuhu embryonic tangata
Kaihautu Whakaaturanga	Tangata
Tūtohu	C-Ko tana tohu
Taupānga	He pai mo te whakamahi i nga immunoassays. Me whakatau e ia taiwhanga he tohu mahi tino pai mo te whakamahi i roto i ana tono.
Nga korero whanui	Ko te CEACAM5 Protein tangata whakahiato ka hangaia e te tangata 293 pūtau (HEK293) me te tohu ira e whakawaehere ana Lys 35 - Ala 685 e whakaatuhia ana me te 6-Tona tohu i te C-terminus.

Maamaa	>95% i whakatauhia e SDS-PAGE.
Te rāpoi ngota Mass	Ko te pūmua he 77.6kDa.Ka heke te pūmua hei 90-130 kDa i raro i te ahua whakaheke (R) (SDS-PAGE) na te rereke o te glycosylation.
Kaipupuri Hua	20 mM PB, 300 mM NaCl, 5% Glycerol, pH 7.4.
Rokiroki	Whakaorangia i raro i nga tikanga parakore i te -20 ℃ ki te -80 ℃ ina whiwhi. Ka tūtohu kia tohatohahia te pūmua ki nga rahinga iti mo te rokiroki pai.