Whakaaturanga Pūmua Prokaryotic
Ko te punaha korero prokaryotic E. coli e tino mohiohia ana he punaha tino whai hua, he maamaa te hangarau, he punaha e whakamahia ana mo te whakaputa pūmua.I Bioantibody, kei te whakapau kaha matou ki te whakarato ki o taatau kaihoko nga ratonga matawhānui, kotahi-mutu mai i te whakahiato ira ki te whakaputa pūmua me te purenga.Kei roto i a maatau ratonga te arotautanga o te codon kore utu me te whakamahi i a maatau hangarau rangatira ki te whakatika i nga take e pa ana ki te iti o te whakapuaki me te koretake ka ara ake i te wa katoa o te whakaaturanga me te purenga.Ko o taatau kaihoko anake te tuku i te ira, te raupapa waikawa amino ranei o te pūmua ka taea e matou te tuku pūmua kounga teitei i roto i te tere mo te toru wiki.I tua atu, ka tukuna e Bioantibody te tango endotoxin me nga ratonga tohu e rite ana ki nga hiahia a o taatau kaihoko.Ka whakapau kaha matou ki te tuku i nga hua me te oati kia kaua e utu he utu mena kaore i te whakaatuhia te pūmua whakamutunga.
Nga Taonga Ratonga | Ihirangi Whakamatau | Wā Arataki(BD) |
Te Hanganga Ira | Ko te arotautanga o te Codon, te whakahiato ira me te whakakorikori. | 5-10 |
Te Whakaaturanga Whakaaturanga me te Taatari Wairewa | 1. Te panoni me te incubation, te kitenga korero me te SDS-PAGE.2. Te tātari wairewa, SDS-PAGE me te kitenga WB | 10 |
He nui te incubation me te purenga, te pūmua whakamutunga (te ma>85%, 90%, 95%) me te ripoata whakamatautau paerewa | Te purenga hononga (Ni tīwae, MBP, GST) |
Mena ka whakahiatotia te ira ki rotoBioantibody, ka whakauruhia te plasmid i hangaia ki nga tuku.