Whakamahinga Whakamahinga
He pai tenei hua mo te tirotiro haumanu kounga o nga tauira serum/plasma/toto katoa mo te kitenga o nga paturopi ki a Leishmania.He whakamatautau ngawari, tere me te kore taputapu mo te tohu o te kala-azar na Leishmania.
Tikanga Whakamatau
Ko tenei hua he immunoassay chromatographic rere taha.Kei roto i te rīpene whakamātautau: 1) he papa conjugate tae burgundy kei roto te recombinant rK39 antigen i honoa ki te colloid koura (Leishmania conjugates) me te rapeti IgG-koura conjugates;2) he riipene nitrocellulose membrane kei roto e rua nga roopu whakamatautau (nga roopu M me G) me tetahi roopu whakahaere (pae C).
Nga rauemi / whakaratohia | Rahi (1 Whakamātautau/Kete) | Rahi(5Whakamātautau/Kete)
| Rahi(25Whakamātautau/Kete)
|
Kete Whakamatau | 1 whakamatautau | 5 whakamatautau | 25 nga whakamatautau |
Kaipupuri | 1 pounamu | 5 pounamu | 25/2 pounamu |
Whakataka | 1 wahi | 5 pcs | 25 pcs |
Peeke Whakaeke Tauira | 1 wahi | 5 pcs | 25 pcs |
Tukua Lancet | 1 wahi | 5 pcs | 25 pcs |
Tohutohu Mo te Whakamahi | 1 wahi | 1 wahi | 1 wahi |
Tiwhikete Whakaaetanga | 1 wahi | 1 wahi | 1 wahi |
Kohikohia te Serum/Plasma/Toto katoa kia tika.
Tena panuihia nga tohutohu i mua i te whakamatautau.I mua i te whakamatautau, tukua te kete whakamatautau, te tauira otinga me te tauira kia taurite ki te mahana (15-30 ℃, 59-86 nekehanga Fahrenheit ranei).
1.Tangohia he ngongo tangohanga mai i te kete me te pouaka whakamatautau mai i te putea kiriata ma te haehae i te kakari.Whakanohoia ki runga i te rererangi whakapae.
2.Whakatuwherahia te peeke pepa konumohe putea tirotiro.Tangohia te kaari whakamatautau ka tuu whakapae ki runga tepu.
3.Whakamahia he pipette tuku, whakawhitia tetahi topata (mo te 20μL) toto maihao/ranei 4μL serum /ranei 4μL plasma/ ranei 4μL toto katoa ki roto i te tauira pai i runga i te rīpene whakamātautau.
4.Whakatuwheratia te ngongo parepare.Whakanohia kia 3 nga pata (tata ki te 80 μL) o te waimeha whakamatautau ki roto i te waimeha whakamatautau te ahua porotaka.Tīmatahia te tatau.
Pānuihia te hua i te 5-10 meneti.Ko nga hua i muri i te 10 meneti ka muhu.
Hua kino
Mena ka puta noa te raina mana kounga C, karekau nga rarangi kitenga G me M e whakaatu
Hua Pai
1.Ko te raina whakahaere kounga C me te raina kitenga M ka puta= ka kitea te antibody Leishmania IgM, a he pai te hua mo te IgM antibody.
2.Ka puta te raina mana kounga C me te raina kitenga G= kua kitea te antibody Leishmania IgG me te hua he pai mo te IgG antibody.
3. Ka puta nga raina mana kounga C me nga rarangi kitenga G me M = nga paturopi Leishmania IgG me IgM.ka kitea, a he pai te hua mo nga paturopi IgG me IgM.
Hua Muhu
Kaore e taea te kite i te raina mana kounga C, ka muhu nga hua ahakoa ka whakaatu mai he raina whakamatautau, me tuku ano te whakamatautau.
Ingoa Hua | ngeru.Kao | Rahi | Tauira | Te Ora Papa | Whakawhiti.& Sto.Temp. |
Leishmania IgG/IgM Antibody Test Kit (Immunochromatographic Assay) | B020C-01 | 1whakamatautau/kete | Serum / Plasma / Toto Katoa | 18 Marama | 2-30 ℃ / 36-86℉ |
B020C-05 | 5 nga whakamatautau / kete | ||||
B020C-25 | 25 nga whakamatautau / kete |